Microscopy:Article Title: Electrophysiological Recording for Study of Retinotectal Circuitry
Article Snippet: Deionized (DI) water Extracellular bath saline Internal recording saline MS-222 (0.02%) (Tricane methanesulfonate; in 1 × Steinberg’s solution) Steinberg’s solution Sylgard-184 (Electron Microscopy Sciences, cat. no. 24236-10) .. Back-projector screen Clampex 10.2 (Molecular Devices) Clampfit 10.2 (Molecular Devices) Digidata 1440A data acquisition system (Molecular Devices) Dissecting stereomicroscope (Nikon, model no. SMZ 1500 or similar) Epifluorescence microscope (equipped with 10× or 20× objective, Nikon Instruments) Forceps (Dumont no. 5; Fine Science Tools) Insect pins Kodak neutral density filters MATLAB (The MathWorks) Microforge (Narishige, MF-830) Micromanipulator controller (e.g., MP-225, Sutter Instrument) Micropipettes with filaments (Borosilicate glass capillaries, Warner Instruments) Micropipette puller (P-97, Sutter Instrument) Recording chamber Patch-clamp amplifier (Multiclamp 700B amplifier, Molecular Devices) Projector (Samsung SP-P310ME LED) Screen luminance meter (SM208, M&A Instruments Inc) ..
Article Title: Electrophysiological Recording for Study of Retinotectal Circuitry
Article Snippet: .. Reagents Deionized (DI) water Extracellular bath saline Internal recording saline MS-222 (0.02%) (Tricane methanesulfonate; in 1 × Steinberg’s solution) Steinberg’s solution Sylgard-184 (Electron Microscopy Sciences, cat. no. 24236-10) Equipment Back-projector screen Clampex 10.2 (Molecular Devices) Clampfit 10.2 (Molecular Devices) Digidata 1440A data acquisition system (Molecular Devices) Dissecting stereomicroscope (Nikon, model no. SMZ 1500 or similar) Epifluorescence microscope (equipped with 10× or 20× objective, Nikon Instruments) Forceps (Dumont no. 5; Fine Science Tools) Insect pins Kodak neutral density filters MATLAB (The MathWorks) Microforge (Narishige, MF-830) Micromanipulator controller (e.g., MP-225, Sutter Instrument) Micropipettes with filaments (Borosilicate glass capillaries, Warner Instruments) Micropipette puller (P-97, Sutter Instrument) Recording chamber Patch-clamp amplifier (Multiclamp 700B amplifier, Molecular Devices) Projector (Samsung SP-P310ME LED) Screen luminance meter (SM208, M&A Instruments Inc) .. Deionized (DI) water Extracellular bath saline Internal recording saline MS-222 (0.02%) (Tricane methanesulfonate; in 1 × Steinberg’s solution) Steinberg’s solution Sylgard-184 (Electron Microscopy Sciences, cat. no. 24236-10)
Patch Clamp:Article Title: Electrophysiological Recording for Study of Retinotectal Circuitry
Article Snippet: Deionized (DI) water Extracellular bath saline Internal recording saline MS-222 (0.02%) (Tricane methanesulfonate; in 1 × Steinberg’s solution) Steinberg’s solution Sylgard-184 (Electron Microscopy Sciences, cat. no. 24236-10) .. Back-projector screen Clampex 10.2 (Molecular Devices) Clampfit 10.2 (Molecular Devices) Digidata 1440A data acquisition system (Molecular Devices) Dissecting stereomicroscope (Nikon, model no. SMZ 1500 or similar) Epifluorescence microscope (equipped with 10× or 20× objective, Nikon Instruments) Forceps (Dumont no. 5; Fine Science Tools) Insect pins Kodak neutral density filters MATLAB (The MathWorks) Microforge (Narishige, MF-830) Micromanipulator controller (e.g., MP-225, Sutter Instrument) Micropipettes with filaments (Borosilicate glass capillaries, Warner Instruments) Micropipette puller (P-97, Sutter Instrument) Recording chamber Patch-clamp amplifier (Multiclamp 700B amplifier, Molecular Devices) Projector (Samsung SP-P310ME LED) Screen luminance meter (SM208, M&A Instruments Inc) ..
Article Title: Electrophysiological Recording for Study of Retinotectal Circuitry
Article Snippet: .. Reagents Deionized (DI) water Extracellular bath saline Internal recording saline MS-222 (0.02%) (Tricane methanesulfonate; in 1 × Steinberg’s solution) Steinberg’s solution Sylgard-184 (Electron Microscopy Sciences, cat. no. 24236-10) Equipment Back-projector screen Clampex 10.2 (Molecular Devices) Clampfit 10.2 (Molecular Devices) Digidata 1440A data acquisition system (Molecular Devices) Dissecting stereomicroscope (Nikon, model no. SMZ 1500 or similar) Epifluorescence microscope (equipped with 10× or 20× objective, Nikon Instruments) Forceps (Dumont no. 5; Fine Science Tools) Insect pins Kodak neutral density filters MATLAB (The MathWorks) Microforge (Narishige, MF-830) Micromanipulator controller (e.g., MP-225, Sutter Instrument) Micropipettes with filaments (Borosilicate glass capillaries, Warner Instruments) Micropipette puller (P-97, Sutter Instrument) Recording chamber Patch-clamp amplifier (Multiclamp 700B amplifier, Molecular Devices) Projector (Samsung SP-P310ME LED) Screen luminance meter (SM208, M&A Instruments Inc) .. Deionized (DI) water Extracellular bath saline Internal recording saline MS-222 (0.02%) (Tricane methanesulfonate; in 1 × Steinberg’s solution) Steinberg’s solution Sylgard-184 (Electron Microscopy Sciences, cat. no. 24236-10)
Saline:Article Title: Electrophysiological Recording for Study of Retinotectal Circuitry
Article Snippet: .. Reagents Deionized (DI) water Extracellular bath saline Internal recording saline MS-222 (0.02%) (Tricane methanesulfonate; in 1 × Steinberg’s solution) Steinberg’s solution Sylgard-184 (Electron Microscopy Sciences, cat. no. 24236-10) Equipment Back-projector screen Clampex 10.2 (Molecular Devices) Clampfit 10.2 (Molecular Devices) Digidata 1440A data acquisition system (Molecular Devices) Dissecting stereomicroscope (Nikon, model no. SMZ 1500 or similar) Epifluorescence microscope (equipped with 10× or 20× objective, Nikon Instruments) Forceps (Dumont no. 5; Fine Science Tools) Insect pins Kodak neutral density filters MATLAB (The MathWorks) Microforge (Narishige, MF-830) Micromanipulator controller (e.g., MP-225, Sutter Instrument) Micropipettes with filaments (Borosilicate glass capillaries, Warner Instruments) Micropipette puller (P-97, Sutter Instrument) Recording chamber Patch-clamp amplifier (Multiclamp 700B amplifier, Molecular Devices) Projector (Samsung SP-P310ME LED) Screen luminance meter (SM208, M&A Instruments Inc) .. Deionized (DI) water Extracellular bath saline Internal recording saline MS-222 (0.02%) (Tricane methanesulfonate; in 1 × Steinberg’s solution) Steinberg’s solution Sylgard-184 (Electron Microscopy Sciences, cat. no. 24236-10)
Electron Microscopy:Article Title: Electrophysiological Recording for Study of Retinotectal Circuitry
Article Snippet: .. Reagents Deionized (DI) water Extracellular bath saline Internal recording saline MS-222 (0.02%) (Tricane methanesulfonate; in 1 × Steinberg’s solution) Steinberg’s solution Sylgard-184 (Electron Microscopy Sciences, cat. no. 24236-10) Equipment Back-projector screen Clampex 10.2 (Molecular Devices) Clampfit 10.2 (Molecular Devices) Digidata 1440A data acquisition system (Molecular Devices) Dissecting stereomicroscope (Nikon, model no. SMZ 1500 or similar) Epifluorescence microscope (equipped with 10× or 20× objective, Nikon Instruments) Forceps (Dumont no. 5; Fine Science Tools) Insect pins Kodak neutral density filters MATLAB (The MathWorks) Microforge (Narishige, MF-830) Micromanipulator controller (e.g., MP-225, Sutter Instrument) Micropipettes with filaments (Borosilicate glass capillaries, Warner Instruments) Micropipette puller (P-97, Sutter Instrument) Recording chamber Patch-clamp amplifier (Multiclamp 700B amplifier, Molecular Devices) Projector (Samsung SP-P310ME LED) Screen luminance meter (SM208, M&A Instruments Inc) .. Deionized (DI) water Extracellular bath saline Internal recording saline MS-222 (0.02%) (Tricane methanesulfonate; in 1 × Steinberg’s solution) Steinberg’s solution Sylgard-184 (Electron Microscopy Sciences, cat. no. 24236-10)
other:Article Title: Scalable ultrasmall three-dimensional nanowire transistor probes for intracellular recording.
Article Snippet: 1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.. 2Advanced Technology Institute, University of Surrey, Guildford, Surrey, UK.. 3Center for Nanomedicine, Institute for Basic Science (IBS), Yonsei-IBS Institute, Yonsei University, Seoul, Republic of Korea.
Software:Article Title: Multiphoton Microscopy
Article Snippet: Using a setup similar to that used in conventional slice recording, gravityfed perfusion medium heated to physiological temperature (~35 °C) using an in-line solution heater (SH-27B; Harvard Apparatus) enters the chamber on one side and is removed by suction at the opposite side, using a low-gauge hypodermic needle set at the desired fluid level and attached to a vacuum line. .. A motorized x-y translating stage (e.g., XYR-AB-4040; Dover Motors) controlled by ScanImage software through a micromanipulator controller (e.g., Sutter MP-285; Sutter Instrument) is highly recommended. ..
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